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pc4 rhe frb fis1  (Addgene inc)


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    Structured Review

    Addgene inc pc4 rhe frb fis1
    Pc4 Rhe Frb Fis1, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pc4+rhe+frb+fis1/pC4-RhE-FRB-Fis1+(Plasmid+%2368056)/pm37851575-221-6-7
    Average 91 stars, based on 3 article reviews
    pc4 rhe frb fis1 - by Bioz Stars, 2026-09
    91/100 stars

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    Related Articles

    Derivative Assay:

    Article Title: A PINK1 input threshold arises from positive feedback in the PINK1/Parkin mitophagy decision circuit.
    Article Snippet: The SNAP tag insert was derived from pSNAPf (NEB, N9183S). .. The FRB-Fis1 insert was derived from pC4-RhE-FRB-Fis1 (Addgene, #68056).64 TheFKBP insertwasderived frompC4M-F2E-GFP-FKBP (Addgene, #68058),64 thePINK1(110–581) insert, lacking the1–109mitochondrial targeting region, was derived from pCMVTNT-PINK1-C-Myc (Addgene, #13314)65 and the mNeonGreen insert was obtained as cDNA (Allele Biotech, ABP-FP-MNEONSB).61 The lentiviral doxycycline-inducible PINK1 expression vector, pLv-TetOn-FKBPPINK1(110–581)-mNeonGreen (Neo), was created using the backbone from pLv-TetOn-MCS1-P2A-MCS2 (Neo) (Addgene, #89180). ..

    Article Title: A PINK1 input threshold arises from positive feedback in the PINK1/Parkin mitophagy decision circuit
    Article Snippet: The SNAP tag insert was derived from pSNAPf (NEB, N9183S). .. The FRB-Fis1 insert was derived from pC4-RhE-FRB-Fis1 (Addgene, #68056). .. The FKBP insert was derived from pC4M-F2E-GFP-FKBP (Addgene, #68058), the PINK1(110–581) insert, lacking the 1–109 mitochondrial targeting region, was derived from pCMVTNT-PINK1-C-Myc (Addgene, #13314) and the mNeonGreen insert was obtained as cDNA (Allele Biotech, ABP-FP-MNEONSB).

    Expressing:

    Article Title: A PINK1 input threshold arises from positive feedback in the PINK1/Parkin mitophagy decision circuit.
    Article Snippet: The SNAP tag insert was derived from pSNAPf (NEB, N9183S). .. The FRB-Fis1 insert was derived from pC4-RhE-FRB-Fis1 (Addgene, #68056).64 TheFKBP insertwasderived frompC4M-F2E-GFP-FKBP (Addgene, #68058),64 thePINK1(110–581) insert, lacking the1–109mitochondrial targeting region, was derived from pCMVTNT-PINK1-C-Myc (Addgene, #13314)65 and the mNeonGreen insert was obtained as cDNA (Allele Biotech, ABP-FP-MNEONSB).61 The lentiviral doxycycline-inducible PINK1 expression vector, pLv-TetOn-FKBPPINK1(110–581)-mNeonGreen (Neo), was created using the backbone from pLv-TetOn-MCS1-P2A-MCS2 (Neo) (Addgene, #89180). ..

    De-Phosphorylation Assay:

    Article Title: PINK 1 phosphorylates Drp1 S616 to regulate mitophagy‐independent mitochondrial dynamics
    Article Snippet: Plasmids encoding PINK1 variants were described 38 . cDNA encoding Drp1 (Human isoform 1) was cloned into pcDNA3.1/myc‐his(B+) (Invitrogen, San Diego, USA). .. The dephosphorylation‐mimic of Drp1 S616 mutants (Drp1 S616A ) was generated using a QuikChange site‐directed Mutagenesis Kit from Stratagene (La Jolla, USA). cDNA encoding Drp1 518‐736 and Drp1 518‐736, S616A were subcloned into pGEX4T‐2GE. pC4M‐F2E‐GFP‐FKBP (68058) and pC4‐RhE‐FRB‐Fis1 (68056) were from Addgene (Cambridge, USA). cDNA encoding ▵110‐PINK1 (WT or D384N) with deletion of N‐terminal 1‐110 aa was subcloned into pC4M‐F2E‐GFP‐FKBP to express ▵110‐PINK1 (WT or D384N)‐GFP‐FKBP. ..

    Article Title: PINK1 phosphorylates Drp1 S616 to regulate mitophagy-independent mitochondrial dynamics.
    Article Snippet: Plasmids encoding PINK1 variants were described [38]. cDNA encoding Drp1 (Human isoform 1) was cloned into pcDNA3.1/myc-his(B+) (Invitrogen, San Diego, USA). .. The dephosphorylation-mimic of Drp1S616 mutants (Drp1S616A) was generated using a QuikChange site-directed Mutagenesis Kit from Stratagene (La Jolla, USA). cDNA encoding Drp1518-736 and Drp1518-736, S616A were subcloned into pGEX4T-2GE. pC4M-F2E-GFPFKBP (68058) and pC4-RhE-FRB-Fis1 (68056) were from Addgene (Cambridge, USA). cDNA encoding M110-PINK1 (WT or D384N) with deletion of N-terminal 1-110 aa was subcloned into pC4M-F2EGFP-FKBP to express M110-PINK1 (WT or D384N)-GFP-FKBP. ..

    Generated:

    Article Title: PINK 1 phosphorylates Drp1 S616 to regulate mitophagy‐independent mitochondrial dynamics
    Article Snippet: Plasmids encoding PINK1 variants were described 38 . cDNA encoding Drp1 (Human isoform 1) was cloned into pcDNA3.1/myc‐his(B+) (Invitrogen, San Diego, USA). .. The dephosphorylation‐mimic of Drp1 S616 mutants (Drp1 S616A ) was generated using a QuikChange site‐directed Mutagenesis Kit from Stratagene (La Jolla, USA). cDNA encoding Drp1 518‐736 and Drp1 518‐736, S616A were subcloned into pGEX4T‐2GE. pC4M‐F2E‐GFP‐FKBP (68058) and pC4‐RhE‐FRB‐Fis1 (68056) were from Addgene (Cambridge, USA). cDNA encoding ▵110‐PINK1 (WT or D384N) with deletion of N‐terminal 1‐110 aa was subcloned into pC4M‐F2E‐GFP‐FKBP to express ▵110‐PINK1 (WT or D384N)‐GFP‐FKBP. ..

    Article Title: PINK1 phosphorylates Drp1 S616 to regulate mitophagy-independent mitochondrial dynamics.
    Article Snippet: Plasmids encoding PINK1 variants were described [38]. cDNA encoding Drp1 (Human isoform 1) was cloned into pcDNA3.1/myc-his(B+) (Invitrogen, San Diego, USA). .. The dephosphorylation-mimic of Drp1S616 mutants (Drp1S616A) was generated using a QuikChange site-directed Mutagenesis Kit from Stratagene (La Jolla, USA). cDNA encoding Drp1518-736 and Drp1518-736, S616A were subcloned into pGEX4T-2GE. pC4M-F2E-GFPFKBP (68058) and pC4-RhE-FRB-Fis1 (68056) were from Addgene (Cambridge, USA). cDNA encoding M110-PINK1 (WT or D384N) with deletion of N-terminal 1-110 aa was subcloned into pC4M-F2EGFP-FKBP to express M110-PINK1 (WT or D384N)-GFP-FKBP. ..

    Mutagenesis:

    Article Title: PINK 1 phosphorylates Drp1 S616 to regulate mitophagy‐independent mitochondrial dynamics
    Article Snippet: Plasmids encoding PINK1 variants were described 38 . cDNA encoding Drp1 (Human isoform 1) was cloned into pcDNA3.1/myc‐his(B+) (Invitrogen, San Diego, USA). .. The dephosphorylation‐mimic of Drp1 S616 mutants (Drp1 S616A ) was generated using a QuikChange site‐directed Mutagenesis Kit from Stratagene (La Jolla, USA). cDNA encoding Drp1 518‐736 and Drp1 518‐736, S616A were subcloned into pGEX4T‐2GE. pC4M‐F2E‐GFP‐FKBP (68058) and pC4‐RhE‐FRB‐Fis1 (68056) were from Addgene (Cambridge, USA). cDNA encoding ▵110‐PINK1 (WT or D384N) with deletion of N‐terminal 1‐110 aa was subcloned into pC4M‐F2E‐GFP‐FKBP to express ▵110‐PINK1 (WT or D384N)‐GFP‐FKBP. ..

    Article Title: PINK1 phosphorylates Drp1 S616 to regulate mitophagy-independent mitochondrial dynamics.
    Article Snippet: Plasmids encoding PINK1 variants were described [38]. cDNA encoding Drp1 (Human isoform 1) was cloned into pcDNA3.1/myc-his(B+) (Invitrogen, San Diego, USA). .. The dephosphorylation-mimic of Drp1S616 mutants (Drp1S616A) was generated using a QuikChange site-directed Mutagenesis Kit from Stratagene (La Jolla, USA). cDNA encoding Drp1518-736 and Drp1518-736, S616A were subcloned into pGEX4T-2GE. pC4M-F2E-GFPFKBP (68058) and pC4-RhE-FRB-Fis1 (68056) were from Addgene (Cambridge, USA). cDNA encoding M110-PINK1 (WT or D384N) with deletion of N-terminal 1-110 aa was subcloned into pC4M-F2EGFP-FKBP to express M110-PINK1 (WT or D384N)-GFP-FKBP. ..

    Plasmid Preparation:

    Article Title: A PINK1 input threshold arises from positive feedback in the PINK1/Parkin mitophagy decision circuit
    Article Snippet: pSNAPf , NEB , N9183S. .. pC4-RhE-FRB-Fis1 , Narendra et al. , Addgene Plasmid #68056. .. pC4M-F2E-GFP-FKBP , Narendra et al. , Addgene Plasmid #68058.



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